sticky ends การใช้

• The DNA is cleaved at the palindromic sequence to produce'sticky ends '.
• These ends can reconnect through hybridization and are termed " sticky ends ".
• Jackie meets a sticky end, when he is stabbed by Terry.
• Essentially, he has created a spaghetti noodle with a sticky end and a repellent end.
• Sticky end links are different in their stability.
• However, they met the same sticky end that they did in 2012, losing 2-0 to the Lynx.
• The problem is that pirates moved from ship to ship and often came to a sticky end,
• Ronald W . Davis first discovered sticky ends as the product of the action of EcoRI, the restriction endonuclease.
• Upon expression, the enzyme will cleave the plasmid at its corresponding recognition site creating sticky ends on the plasmid.
• Blunt-end ligation is much less efficient than sticky end ligation, typically the reaction is 100X slower than sticky-end ligation.
• Also predictions from molecular dynamics simulations show that some sticky end links are much stronger in stretch than the others.
• There is a popular story that Hugh de Grandmesnil almost came to a sticky end at the Battle of Hastings.
• The ends are blunt and can be ligated into a blunt cloning site easily but with lower efficiency than sticky ends.
• PCR usually generates blunt-ended PCR products, but note that PCR using TA ligation is therefore a form of sticky end ligation.
• Ligases then joins the sticky ends to the corresponding sticky ends of the foreign DNA fragments creating a recombinant DNA plasmid.
• Ligases then joins the sticky ends to the corresponding sticky ends of the foreign DNA fragments creating a recombinant DNA plasmid.
• To compensate for the lower efficiency, the concentration of ligase used is higher than sticky end ligation ( 10x or more ).
• "' DNA end "'or "'sticky end "'refers to the properties of the end of a molecule of DNA or a recombinant DNA molecule.
• The sticky end, the dopa portion, adheres to a surface pretty much any surface _ and is hidden by the rest of the noodle.
• A group of students battle the bears in an attempt to save Clevenger, but Clevenger and his wife ultimately come to a sticky end.